Pairwise distances command line: raxmlHPC-PTHREADS.exe -T 2 -f x -n infile.tre -s infile.txt -p 777 -m GTRGAMMA -O RAXMLHPC8_XSEDE-A2AB5A07B7094403B19E213FF014DB73 (saved) produces: raxmlHPC-PTHREADS (T 8) -f x -n outfile.tre -s infile.txt -m GTRGAMMA -p 777 -O RAXMLHPC8_XSEDE select_analysis_=fx (default is fd) outsuffix_=outfile.tre parsimony_seed_val_=362 (set the parsimony value for the starting tree, turned off for -f a or if a tree is supplied using treeetop) dna_gtrcat_=GTRGAMMA (default for datatype_=dna; default for dna_gtrcat_=GTRCAT) disable_seqcheck_=1 (sets the -O value, default is off) Job is OK, command line is raxmlHPC-PTHREADS (-T 8) -f x -n outfile.tre -s infile.txt -p 777 -m GTRGAMMA -O ' Test passed select_analysis: use this to choose the main analysis. if the option is -f a, the value is fa; if -f o, fo, and so forth. other model options: If not DNA , set datatype_=(protein/rna/binary/multi) then set this parameter (GTRCAT default; prot_sub_model_=PROTGAMMA/PROTCAT; rna_model_ ;bin_model_=BINCAT/BINGAMMA; multi_model_=MULTICAT/MULTIGAMMA) Then set sub_parameters for protein: prot_matrix_spec_= (DAYHOFF is default) other modifiers can be set: invariable_= - switch - Estimate proportion of invariable sites (GTRGAMMA + I) ascertainment_= switch - ascertainment_corr_= excl - sets the kind of correction used use_emp_freqs_= to use empirical frequencies, protein only outgroup_ - String - Outgroup (one or more comma-separated outgroups, see comment for syntax) upload modifying files constraint_ - InFile - Constraint (-g) binary_backbone_ - InFile - Binary Backbone (-r) partition_ - InFile - Use a mixed/partitioned model? (-q) exclude_file_ - InFile - Create an input file that excludes the range of positions specifed in this file (-E) printbrlength_ - Switch - Print branch lengths (-k)