ML rapid bootstrap search: One step raxmlHPC-PTHREADS.exe -T 2 -f a -x 177 -k -m PROTGAMMABLOSUM62F -p 739 -N 100 -o 1_Euglena_gracilis -s infile2.txt -n infile2.tre -O NGBW-JOB-RAXMLHPC8_REST_XSEDE-0273B9FB9C58437BBC42EB46DC366418 (saved) raxmlHPC-HYBRID -f a -n outfile.tre -s infile.txt -N 100 -p 362 -m PROTGAMMABLOSUM62F -k -x 12345 -o Cirrospilus_variegatus_BOL_D3753_ABCH_F -O RAXMLHPC8_XSEDE This test: toolId=RAXMLHPC8_XSEDE runtime_=0.50 select_analysis_=fa (default is fd) choose_bootstrap_=x (set rapid boostrapping, otherwise "b") seed_value=12345 (set the boostrap seed, default 12345, so it isnt required to set it) printbrlength_=1 (default is 0 -k option) datatype_=protein (choose the data type, dna is default) prot_sub_model_=PROTGAMMA (PROTCAT is default) prot_matrix_spec_=BLOSUM62 (DAYHOFF is default) use_emp_freqs_=F (default is null) parsimony_seed_val_=362 (set the parsimony value for the starting tree, turned off for -f a or if a tree is supplied using treeetop) choose_bootstop_=specify (the default, otherwise "bootstop" boostrap_value_=100 (100 is default) outgroup_=1_Euglena_gracilis (default is off) outsuffix_=outfile.tre disable_seqcheck_=1 (sets the -O value, default is off) Job is OK, command line is raxmlHPC-HYBRID (-T 32) -f a -x 12345 -k -m PROTGAMMABLOSUM62F -p 362 -N 100 -o 1_Euglena_gracilis -s infile.txt -n outfile.tre -O' Test passed select_analysis: use this to choose the main analysis. if the option is -f a, the value is fa; if -f o, fo, and so forth. other model options: If not DNA , set datatype_=(protein/rna/binary/multi) then set this parameter (GTRCAT default; prot_sub_model_=PROTGAMMA/PROTCAT; rna_model_ ;bin_model_=BINCAT/BINGAMMA; multi_model_=MULTICAT/MULTIGAMMA) Then set sub_parameters for protein: prot_matrix_spec_= (DAYHOFF is default) other modifiers can be set: invariable_= - switch - Estimate proportion of invariable sites (GTRGAMMA + I) ascertainment_= switch - ascertainment_corr_= excl - sets the kind of correction used use_emp_freqs_= to use empirical frequencies, protein only outgroup_ - String - Outgroup (one or more comma-separated outgroups, see comment for syntax) upload modifying files constraint_ - InFile - Constraint (-g) binary_backbone_ - InFile - Binary Backbone (-r) partition_ - InFile - Use a mixed/partitioned model? (-q) exclude_file_ - InFile - Create an input file that excludes the range of positions specifed in this file (-E) printbrlength_ - Switch - Print branch lengths (-k)