ML search: One step or several. raxmlHPC-PTHREADS.exe -T 2 -f d -m PROTGAMMABLOSUM62F -N 1 -O -p 130 -o 1_Euglena_gracilis -s infile2.txt" -n infile2.tre &&raxmlHPC-PTHREADS.exe -T 2 -f J -m PROTGAMMABLOSUM62F -t RAxML_bestTree.infile2.tre" -n sh.infile2.tre -s infile2.txt" exit NGBW-JOB-RAXMLHPC2_WORKFLOW-AB9148F840DE4EF78160B2261009146A (saved) raxmlHPC-HYBRID -s infile.txt -p 12345 -m PROTGAMMABLOSUM62F -k -O -f d -N 10 -n infile.tre && raxmlHPC-PTHREADS -f J -t RAxML_bestTree.infile.tre -p 12345 -n sh.infile.tre -s infile.txt -O -m PROTGAMMABLOSUM62 RAXMLHPC2_WORKFLOW This test: toolId=RAXMLHPC2_WORKFLOW (required) first step: runtime_=0.50 (default) specify_workflow_=MLS (no default) datatype_=protein (default=dna, can be others) prot_sub_model_=PROTGAMMA (no default, a value for the model must be set) prot_matrix_spec_=BLOSUM62 (default is DAYHOFF, many other options) invariable_= (default is null) ascertainment_=ASC_ (default is null; ASC_ turns on the option; invariable and asc are mutually exclusive) use_emp_freqs_=F (default is null, F is the other choice) printbrlength_=1 (print branch lengths, default = 0) outgroup_= (default is no outgroup) disable_seqcheck_=1 (default is 0) workflow step specific: mlsearch_shlike_=1 (find sh-like values, default 0) outsuffix_MLS_=infile (name the output files from bootstrapping step, default=infile) mlsearch_combine_=1 (combine the output files default=0) Commandline is: 'raxmlHPC-HYBRID -f d -m ASC_PROTGAMMABLOSUM62F -N 10 -O -p 12345 -s infile.txt -n infile.tre -k && raxmlHPC-PTHREADS -f J -m ASC_PROTGAMMABLOSUM62 -t RAxML_bestTree.infile.tre -p 12345 -n sh.infile.tre -s infile.txt -O && type RAxML_result.infile.tre* > combined_results.infile.tre' test pass dna_gtrcat_=GTRGAMMA (dataytpe -= dna by default) other model options: If not DNA , set datatype_=(protein/rna/binary/multi) then set this parameter (GTRCAT default; prot_sub_model_=PROTGAMMA/PROTCAT; rna_model_ ;bin_model_=BINCAT/BINGAMMA; multi_model_=MULTICAT/MULTIGAMMA) Then set sub_parameters for protein: prot_matrix_spec_= (DAYHOFF is default) other modifiers can be set: invariable_= - switch - Estimate proportion of invariable sites (GTRGAMMA + I) ascertainment_= switch - ascertainment_corr_= excl - sets the kind of correction used use_emp_freqs_= to use empirical frequencies, protein only outgroup_ - String - Outgroup (one or more comma-separated outgroups, see comment for syntax) upload modifying files constraint_ - InFile - Constraint (-g) binary_backbone_ - InFile - Binary Backbone (-r) partition_ - InFile - Use a mixed/partitioned model? (-q) exclude_file_ - InFile - Create an input file that excludes the range of positions specifed in this file (-E) printbrlength_ - Switch - Print branch lengths (-k)