raxmlHPC-HYBRID -m GTRGAMMA -n boot.tre -s infile.txt -O -x 12345 -N 100 -p 12345 && raxmlHPC-PTHREADS -m GTRGAMMA -n con.tre.tre -J MR -z RAxML_bootstrap.boot.tre ' this workflow succeeds. NGBW-JOB-RAXMLHPC2_WORKFLOW-A7B707F88071411B809DB951E76236FC (saved) raxmlHPC-HYBRID -s infile.txt -N 100 -m GTRGAMMA -p 12345 -O -n boot.tre -x 12345 && raxmlHPC-PTHREADS -m GTRGAMMA -n con.tre.tre -J MR -z RAxML_bootstrap.boot.tre ~ RAXMLHPC2_WORKFLOW This test: toolId=RAXMLHPC2_WORKFLOW_DRAFT runtime_=0.50 select_workflow_=BOOTCON (no default) choose_bootstop_=specify (this is default, can be "bootstop") choose_bootstrap_=x (set rapid bootstrapping, otherwise "b") bootstrap_value=100 (default = 100) datatype_=dna (default, can be others) dna_gtrcat_=GTRGAMMA (no default, a value for the model must be set) outgroup_= (default) provide_parsimony_seed_=1 (default) parsimony_seed_val_=12345 (default) disable_seqcheck_=1 (sets the -O value, default is off) outsuffix_BOOTCON_=boot (sets file name using -n, default = boot) rapidbootstrap_seed_val=12345 (default) outsuffix_BOOTCON2_=con. tre (sets output file name using -n step 2, default = con.tre) rapidbootstrap_seed_val_=12345 (set the bootstrap seed, default 12345, so it isnt required to set it) raxmlHPC-HYBRID (-T 16) -m GTRGAMMA -n boot.tre -s infile.txt -O -x 12345 -N 100 -p 12345 && raxmlHPC-PTHREADS (-T 8) -m GTRGAMMA -n con.tre.tre -J MR -z RAxML_bootstrap.boot.tre' test passed other model options: If not DNA , set datatype_=(protein/rna/binary/multi) then set this parameter (GTRCAT default; prot_sub_model_=PROTGAMMA/PROTCAT; rna_model_ ;bin_model_=BINCAT/BINGAMMA; multi_model_=MULTICAT/MULTIGAMMA) Then set sub_parameters for protein: prot_matrix_spec_= (DAYHOFF is default) other modifiers can be set: invariable_= - switch - Estimate proportion of invariable sites (GTRGAMMA + I) ascertainment_= switch - ascertainment_corr_= excl - sets the kind of correction used use_emp_freqs_= to use empirical frequencies, protein only outgroup_ - String - Outgroup (one or more comma-separated outgroups, see comment for syntax) upload modifying files constraint_ - InFile - Constraint (-g) binary_backbone_ - InFile - Binary Backbone (-r) partition_ - InFile - Use a mixed/partitioned model? (-q) exclude_file_ - InFile - Create an input file that excludes the range of positions specifed in this file (-E) printbrlength_ - Switch - Print branch lengths (-k)