This is a raxml gui command line. The test provides the command line at the bottom of the page. raxmlHPC-PTHREADS.exe -T 2 -f A -t "0" -s infile.txt -m GTRGAMMA -n infile -O -w "C:/Users/mmiller/Desktop/cipres/raxmlgui/DNA_2/" NGBW-JOB-RAXMLHPC8_REST_XSEDE-4B2917C9BA164339A416A27AEA3205EF (saved) raxmlHPC-PTHREADS -f A -n result -s infile.txt -m GTRGAMMA -p 12345 -t tree.tre -O RAXMLHPC8_XSEDE infile_ - Primary Input File runtime_ - Float - Maximum Hours to Run (click here for help setting this correctly) datatype_ - Excl - Sequence Type outgroup_ - String - Outgroup (one or more comma-separated outgroups, see comment for syntax) constraint_ - InFile - Constraint (-g) binary_backbone_ - InFile - Binary Backbone (-r) partition_ - InFile - Use a mixed/partitioned model? (-q) exclude_file_ - InFile - Create an input file that excludes the range of positions specifed in this file (-E) invariable_ - Excl - Estimate proportion of invariable sites (GTRGAMMA + I) prot_matrix_spec_ - Excl - Protein Substitution Matrix empirical_ - Excl - Use empirical base frequencies? mlsearch_ - Switch - Find best tree using maximum likelihood search use_bootstopping_ - Switch - Let RAxML halt bootstrapping automatically (HIGHLY recommended) printbrlength_ - Switch - Print branch lengths (-k) all_outputfiles_ - Results - Job is OK, command line is 'raxmlHPC-PTHREADS (-T 8) -f A -t tree.tre -s infile.txt -m GTRGAMMA -n result -p 12345 -O '