BOXSHADE is a program for pretty-printing multiple alignment output. The program itself doesn't do any alignment, you have to use a multiple alignment program like ClustalW or Pileup and use the output of these programs as input for BOXSHADE. Of course, you can also use manually aligned sequences (in a proper format. INPUT = aligned sequence files
126742336 -------ATGGTGCTGTCTCCCGCTGACAAGGCCAACGTCAAGGCCGCCTGGGGCAAGGT 110005943 ACCCACCATGGTGCTGTCTGCCGCCGACAAGTCCAATGTCAAGGCCGCCTGGGGCAAGGT 66865969 -------ATGGTGCTGTCTGCCGCCGACAAGTCCAATGTCAAGGCCGCCTGGGGCAAGGT ************ **** ****** **** *********************** 126742336 CGGGGGCCACGCCGGCGAGTATGGCGCCGAGGCCCTGGAGAGG----------------- 110005943 TGGCGGCAACGCTGGAGCTTATGGCGCAGAGGCTCTGGAGAGG----------------- 66865969 TGGCGGCAACGCTCCAGCTTTTGGCGCAGAGGCCCTGGAGAGGTGAGCACCGCACCTGCC ** *** **** * * ****** ***** ********* 126742336 ------------------------------------------------------------ 110005943 ------------------------------------------------------------ 66865969 CCGCGGGGATCGGGCCGCACGCCGGGCGCGTCCTTGTCCCGGGCCGCCCGGCCTAAGCCC 126742336 ------------------------ATGTTCCTGAGCTTCCCCACCACCAAGACCTACTTC 110005943 ------------------------ATGTTCCTGAGCTTCCCCACCACCAAGACCTACTTC 66865969 AGTTCTCCTGCCTCTTCACCCAGGATGTTCCTGAGCTTCCCCACCACCAAGACCTACTTC ************************************ 126742336 CCCCACTTCGACGTGACCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAGAAGGTGGCC 110005943 CCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGGCCACGGCGAGAAGGTGGCC 66865969 CCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCGAGAAGGTGGCC ************ *** *********************** ******* *********** 126742336 GATGCCCTCACCCAGGTCGTCGACCACTTGGACGACCTGCCCGGCGCCCTGTCCGCGCTC 110005943 GCCGCGCTGACCAAAGCGGTGGGCCACCTGGACGACCTGCCCGGTACCCTGTCTGATCTG 66865969 AACGCGCTGACCAAAGCGGTGGGCCACCTGGACGATCTGCCCGGTACCCTGTCTGATCTG ** ** *** * * ** * **** ******* ******** ******* * ** 126742336 AGCGACCTGCACGCCCAAAAGCTGCGGGTGGACCCTGTTAACTTCAAG------------ 110005943 AGTGACCTGCACGCCCACAAGCTGCGTGTGGACCCGGTCAACTTCAAG------------ 66865969 AGTGACCTGCACGCTCACAAGCTGCGTGTGGACCCGGTCAACTTCAAGGTGAGCTCGCGG ** *********** ** ******** ******** ** ********* 126742336 ------------------------------------------------------------ 110005943 ------------------------------------------------------------ 66865969 GCTGGGCCGGGACAGATCTGGGCTAGAGGGGCAGAGAATGCGGAGGCGCCCCCACCCAGC 126742336 --------------------------------CTCCTGGCTCACTGCCTGCTGGTGACCC 110005943 --------------------------------CTTCTGAGCCACACCCTGCTGGTGACCC 66865969 CCCAGCCCCCCTGACGTCTCCTCTCTCGGCAGCTTCTGAGCCACACCCTGCTGGTGACCC ** *** *** ************** 126742336 TGGCCAACCACCACCCCAATGAATTCACTCCTGCGGTGCACGCCTCCCTGGACAAGTTCC 110005943 TGGCCTGCCACCTCCCCAATGATTTCACCCCCGCCGTCCACGCCTCCCTGGACAAGTTCT 66865969 TGGCCTCCCACCTCCCCAATGATTTCACCCCCGCGGTCCACGCCTCCCTGGACAAGTTCT ***** ***** ********* ***** ** ** ** ********************* 126742336 TGGCCAACGTGAGCACCGTGCTCACCTCCAAGTATCGTTAA------------------- 110005943 TGGCCAGCGTGGGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGGGCCTCGACGACCC 66865969 TGGCCAACGTGAGCACCGTGCTGACCTCCAAATACCGTTAA------------------- ****** **** ********** ******** ** ****** 126742336 ----------------------------------------------- 110005943 CCTACCCTGGCCTGGAGCGCCCTTGCGCTCTGCGCACTCTCACCTCC 66865969 -----------------------------------------------